Chemical constituents and antioxidants of Lycium barbarum L.
DOI:
https://doi.org/10.32792/utq/utjsci.v8i2.813Keywords:
antioxidant activity, GC-MS, Lycium barbarum, chemical compoundsAbstract
The antioxidant activity of the contents of alkaloid and hexane extracts of lycium barbarum was determined and the percentage of free radical inhibition was calculated using DPPH at concentrations (5,10,25,50,75,100) µg/ml using free radical scavenging activity (DPPH) by adding concentrations Various of extracts to DPPH. The inhibitory activity of DPPH free radicals was determined using six different concentrations (5,10,25,50,75,100) µg /ml of lycium barbarum extracts. The results indicated that the concentration of 100 µg /ml showed stronger radical scavenging activity than the lower concentrations. The results of the percentage of free radical inhibition by calculating the IC50 value of the hexane extract of the lycium barbarum plant showed the strongest with a value of (84.5 mcg/ml) compared with the control agent ascorbic acid (4.29 mcg/ml) followed by the alkaloid extract of the bramble plant (5.74 mcg/ml). The total antioxidant capacity of the test samples was calculated using the standard curve as equivalents of ascorbic acid per gram of extracts, for ascorbic acid (y = 0.04245x + 48.177, R² = 0.3224). The results were for lycium barbarum hexane (y = 0.6118x + 46.425, R² = 0.4849) and alkaloid for bramble (y = 0.6337x + 46.361, R² = 0.5284), respectively.
The antioxidant activity of the contents of alkaloid and hexane extracts of lycium barbarum was determined and the percentage of free radical inhibition was calculated using DPPH at concentrations (5,10,25,50,75,100) µg/ml using free radical scavenging activity (DPPH) by adding concentrations Various of extracts to DPPH.
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