The Effect of Intramuscular Administration of Glutathione as Antioxidant on Semen analysis parameters of Infertile Patients Affected with Oligoasthenozoospermia

Abstract

This study was designed to evaluate the effect of intramuscular administration of Glutathione (100, 200, 300
mg/person) daily for 3 months on seminal fluid analysis parameters prepared by simple layering technique and
centrifugation swim up technique in infertile subjects affected with oligoasthenozoospermia. Thirty infertile men
enrolled in this study and semen samples were analyzed before and after injection with Glutathione and semen
processed by standard semen parameters. Furthermore, sperm concentration, sperm motility, progressive sperm motility,
sperm agglutination, and normal sperm morphology were evaluated according to standard WHO criteria (2010). For
preparation technique, sperm prepared and incubated for 30 minute in 5% CO2 at 37ºC. The results of the present study
indicate a highly significant (P<0.001) differences in all sperm functions after injection with Glutathione in all infertile
patients and concluded that sperm functions improved and enhanced whenever increase the dose of antioxidants and the
simple layering technique gave the best results as compared with centrifugation swim up technique. Further studies are
recommended to assess the detrimental effect of CSUT on sperm DNA damage and embryo quality after in vitro
fertilization and embryo transfer (IVF-ET).