Detection the ability of Uropathogenic bacteria to produce Biofilm andEvaluation the Screening methods

Abstract

twenty urine samples were collected from patients have acute inflammation of the urinary tract with
kidney sands . three types of bacteria were isolated from samples. it was found that the main causative
agent for 85% of these conditions due to E.coli while the remaining ones belongs to Pseudomonas
aeruginosa and Klebsiella pneumoniae. to detect the ability of these isolates to form biofilm . two
different methods were used , the first method is tube method (TM) showed that E. coli possesses
high effectiveness of biofilm formation in the glass as well as Pseudomonas aeruginosa while the
bacterium Klebsiella pneumoniae able to form biofilm moderately. It was observed when using the
second method Congo red method (CRA) that all isolates composed biofilm when cultured on CRA
except 5% of isolates belongs to bacterium Pseudomonas aeruginosa was negative for this test, . the
first method is more accurate than the other to show the degree of biofilm formation to the naked eye, and both methods are considered qualitative not quantitative. The present study aims to investigate the
ability of bacteria isolated from people with frequent urinary tract infections UTI associated with
kidney stones on biofilm production .