In Vivo Study: An Innovative Spasm Simulation Utilizing Lactic Acid and Treatment with Baclofen loaded Transferosomal Gel
DOI:
https://doi.org/10.32792/utq/utjsci/v13i1.1464Keywords:
Lactic Acid, Transferosomal Baclofen Gel, Muscles Spasm, Carpobol-934Abstract
Baclofen, a GABA_B receptor agonist, is commonly used to manage muscle spasticity. Although systemic administration is effective, its use is limited by adverse effects. Topical application offers targeted delivery to affected muscles, potentially reducing spasticity while minimizing systemic side effects. To develop an innovative in vivo model for simulating muscle spasms using lactic acid, formulate a baclofen-loaded transferosomal gel suitable for topical application, and investigate its therapeutic efficacy in alleviating muscle spasms in the in vivo model. Twenty male rabbits (Lepus domestica) were divided into four groups. Muscle spasms were induced by intramuscular injection of 30 m Molar lactic acid. Baclofen nanoliposomes were incorporated into a Carbopol 934 gel for topical administration. Zeta potential of the formulation was determined, and the following clinical parameters were measured: lactate dehydrogenase (LDH), creatine kinase (CK), calcium ion (Ca²⁺), potassium ion (K⁺), phosphorus ion (P), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea, and serum creatinine. Baclofen nanoliposomes coated with Carbopol exhibited a high negative zeta potential (−63.3 mV). Significant differences (P < 0.05) were observed in LDH, CK, AST, ALT, Ca²⁺, K⁺, P, blood urea, and serum creatinine between the spasm-induced group and the baclofen gel–treated group. This study provides the first evidence of the safety and efficacy of topical baclofen gel in reducing muscle spasms, supporting its potential in nano-liposomal drug delivery applications and paving the way for future innovations in targeted spasm therapy.
Received: 18-08-2025
Revised: 15-09-2025
Accepted: 13-10-2025
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