Evaluation of Vitamin D and Some Liver Enzymes in Iraqi Colorectal Cancer Patients and those Without Colorectal Cancer
DOI:
https://doi.org/10.32792/utq/utjsci/v13i1.1443Keywords:
biomarkers, CLIA, colorectal cancer, liver enzyme, vitamin DAbstract
Abstract—Colorectal cancer (CRC) is a major global health concern, described as the uncontrolled growing of cells in the colon or rectum. Vitamin D, metabolized in the liver and has garnered attention for its potential protective effects against CRC. The aim of this research is to evaluate vitamin D levels and its link with some liver enzymes in Iraqi participants with colorectal cancer. Overall, 70 colorectal cancer cases-divided into 35 newly diagnosed and 35 under medication were included, along with 30 individuals without colorectal cancer as controls. Blood sample were collected from Iraqi CRC patients and controls. Serum levels of vitamin D and liver biomarkers were analyzed using Cobas C311 and Chemiluminescence Immunoassay technology. The result showed significant variances in vitamin D levels between the studied groups (p=0.0001), with an inverse relationship between vitamin D and alkaline phosphatase (r=-0.33, p=0.0007). This study suggests that vitamin D could have a potential regulatory role in colorectal cancer patients.
Received: 09-07-2025
Revised: 05-08-2025
Accepted: 19-08-2025
References
[1] R. L. Siegel, A. N. Giaquinto, and A. Jemal, "Cancer statistics, 2024," CA: a cancer journal for [1] A. N. M. Ali and S. N. Muslim, "Breast Cancer in Iraq: A Comprehensive Review of Disease Burden, Risk Factors, and Healthcare Response," South Asian Res J Bio Appl Biosci, vol. 7, pp. 349-360, 2025.
clinicians, vol. 74, pp. 12-49, 2024.
[2] A. N. M. Ali and S. N. Muslim, "Breast Cancer in Iraq: A Comprehensive Review of Disease Burden, Risk Factors, and Healthcare Response," South Asian Res J Bio Appl Biosci, vol. 7, pp. 349-360, 2025.
[3] N. A. Alwan, F. Lami, M. Al Nsoor, and D. Kerr, "Trends in the Incidence and Mortality of the Most Common Cancers in Iraq (Iraqi Cancer Registry 1999-2019)," The Gulf Journal of Oncology, vol. 1, pp. 47-57, 2022.
[4] S. Ibrahem, H. Ahmed, and S. Zangana, "Trends in colorectal cancer in Iraq over two decades: incidence, mortality, topography and morphology," Annals of Saudi Medicine, vol. 42, pp. 252-261, 2022.
[5] B. F. Mohammad and I. I. Andsoy, "Health behaviors, knowledge, screening, and attitudes toward colorectal cancer among Iraqi adults," Public Health Nursing, vol. 41, pp. 514-524, 2024.
[6] S. Christakos, P. Dhawan, A. Porta, L. J. Mady, and T. Seth, "Vitamin D and intestinal calcium absorption," Molecular and cellular endocrinology, vol. 347, pp. 25-29, 2011.
[7] H. F. DeLuca, "Overview of general physiologic features and functions of vitamin D," The American journal of clinical nutrition, vol. 80, pp. 1689S-1696S, 2004.
[8] S. Van Cromphaut, K. Rummens, I. Stockmans, E. Van Herck, F. Dijcks, A. Ederveen, et al., "Intestinal calcium transporter genes are upregulated by estrogens and the reproductive cycle through vitamin D receptor‐independent mechanisms," Journal of Bone and Mineral Research, vol. 18, pp. 1725-1736, 2003.
[9] S. Y. Na, K. B. Kim, Y. J. Lim, and H. J. Song, "Vitamin D and Colorectal Cancer: Current Perspectives and Future Directions," J Cancer Prev, vol. 27, pp. 147-156, Sep 30 2022.
[10] E. Yassıbaş, H. İ. Önen, M. Kerem, O. Toka, and G. Samur, "Vitamin D Receptor Gene Polymorphism and Serum Vitamin D Levels with the Risk of Colorectal Cancer: An Observational Case-Control Study," p. 16, 2022.
[11] Q.-E. Ling, S.-Q. Ni, Y. Jiao, and L.-X. Liu, "Unlocking the sun’s potential: Vitamin D’s role in battling colorectal cancer," Journal of Nutritional Oncology, vol. 9, pp. 1-9, 2024.
[12] A. Ottaiano, S. Facchini, M. Santorsola, G. Nasti, G. Facchini, L. Montella, et al., "Circulating vitamin D level and its impact on mortality and recurrence in stage III colorectal cancer patients: a systematic review and meta-analysis," Cancers, vol. 15, p. 3012, 2023.
[13] L. Wang, R. Xu, M. Wang, M. Wang, S. Su, Y. Nian, et al., "Exploration and Identification of Vitamin D and Related Genes as Potential Biomarkers for Colorectal Tumors," OncoTargets and Therapy, vol. 18, pp. 129-145, 2025.
[14] G. Wagener, Liver anesthesiology and critical care medicine: Springer Cham, 2018.
[15] M. m. He, Z. Fang, D. Hang, F. Wang, G. Polychronidis, L. Wang, et al., "Circulating liver function markers and colorectal cancer risk: A prospective cohort study in the UK Biobank," International journal of cancer, vol. 148, pp. 1867-1878, 2021.
[16] J. T. Keane, H. Elangovan, R. A. Stokes, and J. E. Gunton, "Vitamin D and the liver—correlation or cause?," Nutrients, vol. 10, p. 496, 2018.
[17] D. George and P. Mallery, IBM SPSS statistics 29 step by step: A simple guide and reference: Routledge. New York, 2019.
[18] A. K. Fenercioglu, "The Anti-Inflammatory Roles of Vitamin D for Improving Human Health," Current Issues in Molecular Biology, vol. 46, pp. 13514-13525, 2024.
[19] X. Zhang, Y. He, X. Li, R. Shraim, W. Xu, L. Wang, et al., "Circulating 25-hydroxyvitamin D and survival outcomes of colorectal cancer: evidence from population-based prospective cohorts and Mendelian randomisation," British journal of cancer, vol. 130, pp. 1585-1591, 2024.
[20] G. Ferrer-Mayorga, A. Muñoz, and J. M. González-Sancho, "Vitamin D and colorectal cancer," in Feldman and Pike's Vitamin D, ed: Elsevier, 2024, pp. 859-899.
[21] D. Lowe and S. John, Alkaline Phosphatase: StatPearls Publishing LLC, 2019.
[22] T. Gür, H. Demir, and M. Ç. Kotan, "Tumor markers and biochemical parameters in colon cancer patients before and after chemotherapy," Asian Pacific Journal of Cancer Prevention, vol. 12, pp. 3147-50, 2011.
[23] M. W. Saif, D. Alexander, and C. M. Wicox, "Serum alkaline phosphatase level as a prognostic tool in colorectal cancer: a study of 105 patients," The journal of applied research, vol. 5, pp. 88-95, 2005.
[24] O. Ojo, M. Asaolu, O. A. Akinlua, and A. Atiba, "Serum marker enzymes activities in cancer patients," Journal of Chemistry and Biochemistry, vol. 4, pp. 15-21, 2016.
[25] M. J. Hasan, M. M. Bakheet, H. H. Ali, M. K. A. Al-Saedi, and M. Q. Mohammed, "Dual Role of miR-150 in Colorectal Cancer Progression: A Quantitative Real-Time PCR Study," Al-Rafidain Journal of Medical Sciences, vol. 8, pp. 221-229, 2025.
[26] H. A. Rajab, "The effect of vitamin D level on parathyroid hormone and alkaline phosphatase," Diagnostics, vol. 12, p. 2828, 2022.
[27] T. Ao, J. Kikuta, and M. Ishii, "The effects of vitamin D on immune system and inflammatory diseases," Biomolecules, vol. 11, p. 1624, 2021.
[28] C.-Y. Yang, P. S. Leung, I. E. Adamopoulos, and M. E. Gershwin, "The implication of vitamin D and autoimmunity: a comprehensive review," Clinical reviews in allergy & immunology, vol. 45, pp. 217-226, 2013.
[29] M. H. El Gayar, K. M. Makboul, L. M. Hindawy, and A. M. Saleh, "Study of the relationship between colorectal cancer and vit D defficiency," Egyptian Journal of Obesity, Diabetes and Endocrinology, vol. 2, pp. 61-66, 2016.
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