Hospital-Acquired Staphylococcus aureus in Thi-Qar Governorate: A Molecular and Epidemiological Perspective
DOI:
https://doi.org/10.32792/utq/utjsci/v12i2.1418Keywords:
S. aureus, Nosocomial infections, 16S rRNA, PCRAbstract
Staphylococcus aureus (S. aureus) is a pathogenic strain of bacteria, that resists multiple multi-antibiotics used for common bacterial infections. Most S. aureus infections occur among people consulting hospitals or other healthcare settings such as nursing homes and dialysis centers. This study aimed to molecularly identify S. aureus strains associated with nosocomial infections in hospitals of Thi-Qar. A total of 100 samples were collected from various locations in three hospitals in Thi-Qar Governorate. Polymerase Chain Reaction (PCR) technique was utilized to assay the presence of S. aureus via 16S rRNA gene. The study population consisted of both male and female patients, with a male predominance (82% males vs. 18% females), showing statistically significant differences (p ≤ 0.01). The highest frequency of isolates (65%) was observed in the 21–31 age group, which also demonstrated a significant difference compared to other age groups. The PCR amplification of the 16S rRNA gene was successfully submitted to GenBank and assigned the accession numbers LC866513, LC866514, and LC866515. Phylogenetic analysis using MEGA-10 software revealed distinct molecular relationships between the local S. aureus isolates and genetically related strains from various regions worldwide.
The outcomes of this study provide valuable insights that could inform and enhance infection control practices, thereby contributing to the mitigation of disease transmission within healthcare environments.
Received: 2025-05-27
Revised: 2025-06-26
Accepted: 2025-07-12
References
G.Gherardi, "Staphylococcus aureus Infection: Pathogenesis and Antimicrobial Resistance," (in eng), Int J Mol Sci, vol. 24, no. 9, May 3 2023, doi: 10.3390/ijms24098182.
M. Dadashi, B. Hajikhani, D. Darban-Sarokhalil, A. van Belkum, and M. Goudarzi, “Mupirocin resistance in Staphylococcus aureus: A systematic review and meta-analysis,” J. Glob. Antimicrob. Resist., vol. 20, pp. 238–247, 2020.
A. H. Siddiqui and J. Koirala, "Methicillin-Resistant Staphylococcus aureus," in StatPearls. Treasure Island (FL) ineligible companies. Disclosure: Janak Koirala declares no relevant financial relationships with ineligible companies.: StatPearls Publishing, LLC., 2025.
A. R. Collaborators, “Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis,” Lancet, vol. 399, no. 10325, pp. 629–655, 2022.
R. Adame-Gómez et al., "Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals," (in eng), Int J Microbiol, vol. 2020, p. 1048097, 2020, doi: 10.1155/2020/1048097.
J. M. Ngoi et al., "Genomic analysis of SARS-CoV-2 reveals local viral evolution in Ghana," (in eng), Exp Biol Med (Maywood), vol. 246, no. 8, pp. 960-970, Apr 2021, doi: 10.1177/1535370220975351.
E. Worku, D. A. Fenta, and M. M. Ali, "Bacterial etiology and risk factors among newborns suspected of sepsis at Hawassa, Ethiopia," (in eng), Sci Rep, vol. 12, no. 1, p. 20187, Nov 23 2022, doi: 10.1038/s41598-022-24572-0.
A. Y. Alemu, A. Endalamaw, D. M. Belay, D. K. Mekonen, B. M. Birhan, and W. A. Bayih, "Healthcare- associated infection and its determinants in Ethiopia: A systematic review and meta-analysis," (in eng), PLoS One, vol. 15, no. 10, p. e0241073, 2020, doi: 10.1371/journal.pone.0241073.
N. A. Nimer, "Nosocomial Infection and Antibiotic-Resistant Threat in the Middle East," (in eng), Infect Drug Resist, vol. 15, pp. 631-639, 2022, doi: 10.2147/idr.S351755.
S. M. Harman Deep Kaur M, Sehgal MM, Shubham M. , "Assessing the knowledge regarding prevention of nosocomial infection among B. Sc. Nursing 1st year students of Akal College of Nursing, Baru Sahib. ," Int J All Res Educ Scientific Methods no. 8, pp. 1141-7, 2021.
A. Saud and A. Ali, "Assessment of Nurse's Knowledge and Practices about Personal Protective Equipment in Hemodialysis Unit in Basra Teaching Hospital," vol. 15, p. 1351, 10/19 2021, doi: 10.37506/ijfmt.v15i3.15496.
A. Monteiro et al., "Exposure and Health Effects of Bacteria in Healthcare Units: An Overview," Applied Sciences, vol. 12, no. 4, p. 1958, 2022. [Online]. Available: https://www.mdpi.com/2076-3417/12/4/1958.
J. R. Alzaidi, & Mohammed, A. S. , "First record of dissemination of BLBLI-resistant Enterobacter cloacae from Public Hospitals in Baghdad, Iraq. ," The Open Microbiology Journal, vol. 16, no. 1, pp. 1-8, 2022.
A. P. Kourtis et al., "Vital Signs: Epidemiology and Recent Trends in Methicillin-Resistant and in Methicillin- Susceptible Staphylococcus aureus Bloodstream Infections - United States," (in eng), MMWR Morb Mortal Wkly Rep, vol. 68, no. 9, pp. 214-219, Mar 8 2019, doi: 10.15585/mmwr.mm6809e1.
M. A. Ehmud, A. S. Mteran, M. H. Al-Furaiji and G. S. Hasson "Identification and Characterization of Staphylococcus aureus16s rRNA Gene Isolate from Environmental and Clinical Sources", Asian Journal of Dairy and Food Research, vol. 3, p. 1-8, 2025.
E. Reem, J. Douek, and B. Rinkevich, "Historical navigation routes in European waters leave their footprint on the contemporary seascape genetics of a colonial urochordate," Scientific Reports, vol. 13, no. 1, p. 19076, 2023/11/04 2023, doi: 10.1038/s41598-023-46174-0.
G. Tarzi, O. Mendoza, Y. Lunsky, and L. St John, "The experiences and attitudes of family caregivers of adults with intellectual and developmental disabilities at different timepoints in the COVID-19 pandemic," (in eng), Disabil Health J, vol. 16, no. 3, p. 101472, Jul 2023, doi: 10.1016/j.dhjo.2023.101472.
M. C. Basil et al., "Human distal airways contain a multipotent secretory cell that can regenerate alveoli," (in eng), Nature, vol. 604, no. 7904, pp. 120-126, Apr 2022, doi: 10.1038/s41586-022-04552-0.
A. K. Mohammed, “Contamination with Proteus and Staphylococcus spp. effect on physical properties of sheep semen,” Egyptian Journal of Veterinary Sciences, vol. 55, no. 6, pp. 1513–1518, 2024.
G. H. Hussein and R. K. Mohammed, “The prevalence of enterotoxin SEA and SEB genes in Staphylococcus aureus multidrug-resistant isolates from clinical specimens in Baghdad Province,” Iraqi J. Sci., pp. 6476–6484, 2024.
G. H. Hussein and R. K. Mohammed, “The prevalence of enterotoxin SEA and SEB genes in Staphylococcus aureus multidrug-resistant isolates from clinical specimens in Baghdad Province,” Iraqi Journal of Science, vol. 65, no. 11, pp. 6476–6484, 2024. doi: 10.24996/ijs.2024.65.11.24.
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