Molecular and Serological Detection of Helicobacter Pylori Bacteria in Patients with Gastrointestinal Tract Infections
DOI:
https://doi.org/10.32792/utq/utjsci/v12i2.1411Keywords:
Helicobacter pylori, PcR ,VacA,CagA,Serological testsAbstract
Helicobacter pylori is closely associated with gastrointestinal diseases, which are among the most common health problems worldwide. This study aimed to detect H. pylori using serological and molecular diagnostic methods to enhance the accuracy and reliability of testing. Samples were collected from the Digestive System Center/Imam Hussein Teaching Hospital in Thi Qar between October 2024 and March 2025. These included 115 patients of both sexes and ages with gastrointestinal diseases. Serological and molecular tests were performed to detect H. pylori. 45 stool samples were collected; antigen testing was performed, and 20 samples (44.4%) were positive. These samples were then molecularly diagnosed by polymerase chain reaction (PCR), with the following results: H. pylori infection was diagnosed using PCR targeting 16sRNA in 3 out of 20 stool samples (15%). The diagnosis was also confirmed by detecting the virulence factors VacAs1/s2 and CagA, with VacAs1/s2 detected in 3(100%) and CagA in 2(66.66%) patients with gastrointestinal disease. Seventy blood samples were collected, and the H. pylori detection rate using antibody testing was 31(44.28%). The results of this study demonstrated that combining serological and molecular diagnostic methods improves the accuracy of H. pylori detection in patients.
Received: 2025-05-23
Revised: 2025-07-10
Accepted: 2025-07-24
References
H. S. M. Aljaberi, N. K. Ansari, M. Xiong, H. Peng, B. He, and S. Wang, “Current understanding of the transmission, diagnosis, and treatment of H. pylori infection: A comprehensive review,” Int. J. Med. Pharm. Drug Res., vol. 7, 2023.
S. H. Gillespie and P. M. Hawkey, Principles and Practice of Clinical Bacteriology, 3rd ed. Hoboken, NJ: Wiley-Blackwell, 2021, pp. 215–220.
S. Shah, K. Cappell, R. Sedgley, C. Pelletier, R. Jacob, M. Bonafede, and R. Yadlapati, “Diagnosis and treatment patterns among patients with newly diagnosed Helicobacter pylori infection in the United States 2016–2019,” Sci., 2023.
S. DuBois and D. J. Kearney, “Iron-deficiency anemia and Helicobacter pylori infection: A review of the evidence,” American Journal of Gastroenterology, vol. 100, pp. 453–459, 2005.
C. W. Huh and B. W. Kim, “—,” The Korean Journal of Gastroenterology = Taehan Sohwagi Hakhoe chi, vol. 72, no. 5, pp. 229–236, 2018. doi: 10.4166/kjg.2018.72.5.229.
Y. K. Wang, F. C. Kuo, C. J. Liu, M. C. Wu, H. Y. Shih, S. S. Wang, J. Y. Wu, C. H. Kuo, Y. K. Huang, and D. C. Wu, “Diagnosis of Helicobacter pylori infection: Current options and developments,” World Journal of Gastroenterology, vol. 21, no. 40, pp. 11221–11235, 2015. doi: 10.3748/wjg.v21.i40.11221.
M. K. Saeed, B. A. Al-Ofairi, M. A. Hassan, M. A. Al-Jahrani, and A. M. Abdulkareem, “Diagnostic value of IgG antibody and stool antigen tests for chronic Helicobacter pylori infections in Ibb Governorate, Yemen,” Scientific Reports, vol. 14, no. 1, pp. 1–12, 2024. doi: 10.1038/s41598-024-59994-1.
A. F. Cutler and V. M. Prasad, “Long-term follow-up of Helicobacter pylori serology after successful eradication,” American Journal of Gastroenterology, 1996.
J. P. Gisbert, F. de la Morena, and V. Abraira, “Accuracy of monoclonal stool antigen test for the diagnosis of H. pylori infection: A systematic review and meta-analysis,” American Journal of Gastroenterology, 2006.
N. Almeida, M. M. Donato, J. M. Romãozinho, C. Luxo, O. Cardoso, M. A. Cipriano, C. Marinho, A. Fernandes, and C. Sofia, “Correlation of Helicobacter pylori genotypes with gastric histopathology in the central region of a South-European country,” Digestive Diseases and Sciences, 2015.
R. M. Ferreira, J. C. Machado, and C. Figueiredo, “Clinical relevance of Helicobacter pylori vacA and cagA genotypes in gastric carcinoma,” Best Practice & Research: Clinical Gastroenterology, 2014.
L. A. Sicinschi, P. Correa, L. E. Bravo, R. M. Peek, K. T. Wilson, J. T. Loh, et al., “Non-invasive genotyping of Helicobacter pylori cagA, vacA, and hopQ from asymptomatic children,” Helicobacter, 2012.
L. J. Xiong, Y. Tong, Z. Wang, and M. Mao, “Detection of clarithromycin-resistant Helicobacter pylori by stool PCR in children: A comprehensive review of literature,” Helicobacter, 2013. doi: 10.1111/hel.12016.
A. Qasim and H. A. Salih, “Isolation and identification of Klebsiella pneumoniae from wound infections and study of the synergistic effect of carbapenems and cefepime,” University of Thi-Qar Journal of Science, vol. 11, no. 2, pp. 136–141, Dec.2024.
D. I. Grove, R. A. B. McLeay, K. E. Byron, and G. Koutsouridis, “Isolation of Helicobacter pylori after transport from a regional laboratory of gastric biopsy specimens in saline, Portagerm pylori or cultured on chocolate agar,” Pathology, vol. 33,no.3,pp.362–364,2001.
U. Harrison, M. A. Fowora, A. T. Seriki, E. Loell, S. Mueller, M. Ugo-Ijeh, et al., “Helicobacter pylori strains from a Nigerian cohort show divergent antibiotic resistance rates and a uniform pathogenicity profile,” PLOS ONE, vol. 12, no. 5, pp. 1–16, 2017.
J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press,2001,pp.514–518.
P. D. Majeed, K. J. Saleh, and H. M. Abdullah, “Detection of Helicobacter pylori antigens among patients with gastroenteritis in Erbil City, Iraq,” Polytechnic Journal, vol. 9, no. 2, pp. 138–143, 2019.
R. A. Hussein, M. T. S. Al-Ouqaili, and Y. H. Majeed, “Detection of Helicobacter pylori infection by invasive and non-invasive techniques in patients with gastrointestinal diseases from Iraq: A validation study,” PLOS ONE, vol. 16, no. 8, pp. 1–12, Aug.2021.
S. I. Smith, M. A. Fowora, O. A. Lesi, E. Agbebaku, P. Odeigah, F. B. Abdulkareem, C. A. Onyekwere, C. A. Agomo, and M. Contreras, “Application of stool-PCR for the diagnosis of Helicobacter pylori from stool in Nigeria – a pilot study,” Springer Plus, vol. 1, no. 1, p. 78, 2012.
M. H. Radhi and G. J. Mohammed, “Genotyping and virulence genes of Helicobacter pylori isolated from patients with gastroenteritis in Al-Diwaniyah Province/Iraq,” International Journal of Health Sciences, vol. 6, no. S1, pp. 11963–11974, 2022.
J. C. Luo, “Noninvasive diagnostic methods for Helicobacter pylori infection,” Journal of the Chinese Medical Association, vol. 78, no. 2, pp. 83–84, 2015.
H. J. Ali, Immunogenetic study of H. pylori among patients with coronary heart disease, M.Sc. thesis, University of Al-Qasim Green,Iraq,2021,p.65.
S. K. Patel, C. B. Pratap, A. K. Jain, A. K. Gulati, and G. Nath, “Diagnosis of Helicobacter pylori: What should be the gold standard?,” World Journal of Gastroenterology, vol. 20, no. 36, pp. 1284712859,2014.[Online].Available:https://t.me/c/2175951600/71.
P. Malfertheiner, F. Megraud, C. A. O’Morain, J. P. Gisbert, E. J. Kuipers, A. T. Axon, F. Bazzoli, A. Gasbarrini, J. Atherton, D. Y. Graham, R. Hunt, P. Moayyedi, T. Rokkas, M. Rugge, M. Selgrad, S. Suerbaum, K. Sugano, E. M. El-Omar, L. P. Andersen, et al., “Management of Helicobacter pylori infection – The Maastricht IV/Florence consensus report,” Gut, vol. 61, no. 5, pp. 646–664,2012.doi:10.1136/gutjnl-2012-302084.
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