Isolation and identification of bacterial contamination of raw meat in Thi-Qar province

Abstract

Meat considers a favorite environment for bacterial growth due to rich composition with protein, fats, minerals,
vitamins and other essential materials requirements for microorganism growth. Study concluded isolation and bacterial account of meat in Thiqar city markets. Study extends from November2014- March 2015. Thirty samples of meat 10 from beef meat, 10 from sheep and 10 from poultry meat). Bacterial account shown that the highest contamination of meat samples found in sheep meat 16*106 CUF/g (more than standard parameters of healthy suitable meat for human consumption) while the lowest bacterial account found in poultry meat 93*102 CUF/g. Isolation and identification of bacteria by morphological, differential media, biochemical tests and Gram stain results showed that prodomenet bacteria were E.coli, Klebseilla, Staphylococcus aureas, Shegilla, Salmonella spp., Bacillus cereus, Enterobacter, Proteus, Micrococcus, Pseudomonas spp. and streptococcus spp. The predominant bacteria was E. coli about 35% in all samples, Followed by staphylococcus spp. 25%. Salmonella spp.1.2% isolated only from chicken meat samples.