Morphological and Molecular detection of some Opportunistic Free- living Amoebae Isolated From Environmental and clinical Sources in Thi- Qar province / Iraq
DOI:
https://doi.org/10.32792/utq/utjsci/v11i1.1184Keywords:
Free-living amoebas , environmental samples ,Clinical samples ,Thi-qar provinceAbstract
Free- living amoebae (FLA) are predatory heterotrophic protozoa that are widely distributed in various environmental sources .They are known to cause serious human infections including a fatal encephalitis, pneumonia and a blinding keratitis. was performed in Thi –Qar province south of Iraq in order to detection of the opportunistic FLA and identified by morphological characters in the culture , Polymers Chain Reaction (PCR) amplification a targeting specific genes for each genus
During the period from February to September 2020, one hundred and two (102)samples were collected including 75samples from different environmental sources, soil , water ( rivers , tap water , tank water, stagnant water, the marshes water and drops of water from the air conditioner units ) and animals wastes(lizard , birds and wild mice) ,and 27 clinical samples ( eye, skin, ear, and Cerebral Spinal Fluid)collected from Al-Hussain teaching hospital , Bint AL-Huda teaching hospital , Al-Hboobi teaching hospital and private laboratories in Thi-Qar province . All samples were cultured in the Non Nutrient agar medium (NN-agar medium) and then examined by light microscope and recognized morphologically the trophozoites and cysts of opportunistic amoebas that belong to the genera ; Naegleria fowleri, Acanthamoeba spp.(A. triangularis ,A. astronyxis ,A. castellini A. polyphaga ), Sappinia spp.(S. pedata , S. diploidea) and Balamuthia mandrillaris, the study found 62 (60.78%) samples were positive for total opportunistic FLA, and 40(39.21%) samples were negative, the incidence of FLA in environmental samples were 76% and in clinical samples were18.51 % .
Received: 2024-03-03
Revised: 2024-03-19
Accepted: 2024-03-22
References
A. M. Spagnolo, M. Sartini, D. Di Cave, B. Casini, B. Tuvo, and M. L. Cristina, "Evaluation of microbiological and free-living protozoa contamination in dental unit waterlines," International Journal of Environmental Research and Public Health, vol. 16, p. 2648, 2019.
M. Lalle, E. Pozio, G. Capelli, F. Bruschi, D. Crotti, and S. M. Cacciò, "Genetic heterogeneity at the β-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes," International journal for parasitology, vol. 35, pp. 207-213, 2005.
K. Król-Turmińska and A. Olender, "Human infections caused by free-living amoebae," Annals of Agricultural and Environmental Medicine, vol. 24, 2017.
G. S. Visvesvara, H. Moura, and F. L. Schuster, "Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea," FEMS Immunology & Medical Microbiology, vol. 50, pp. 1-26, 2007.
N. A. Khan, "Acanthamoeba: biology and increasing importance in human health," FEMS microbiology reviews, vol. 30, pp. 564-595, 2006.
F. Page, "A new key to freshwater and soil gymnamoebae Freshwater Biological Association," Ambleside, Cumbria, United Kingdom ρ, pp. 14-15, 1988.
J. M. Schroeder, G. C. Booton, J. Hay, I. A. Niszl, D. V. Seal, M. B. Markus, et al., "Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge," Journal of Clinical Microbiology, vol. 39, pp. 1903-1911, 2001.
M. Schild, C. Gianinazzi, B. Gottstein, and N. Müller, "PCR-based diagnosis of Naegleria sp. infection in formalin-fixed and paraffin-embedded brain sections," Journal of Clinical Microbiology, vol. 45, pp. 564-567, 2007.
G. C. Booton, J. R. Carmichael, G. S. Visvesvara, T. J. Byers, and P. A. Fuerst, "Identification of Balamuthia mandrillaris by PCR assay using the mitochondrial 16S rRNA gene as a target," Journal of Clinical Microbiology, vol. 41, pp. 453-455, 2003.
Y. Qvarnstrom, A. J. Da Silva, F. L. Schuster, B. B. Gelman, and G. S. Visvesvara, "Molecular confirmation of Sappinia pedata as a causative agent of amoebic encephalitis," The Journal of infectious diseases, vol. 199, pp. 1139-1142, 2009.
A. Leońska-Duniec, B. Skotarczak, and M. Adamska, "Molecular identification of free-living amoebae isolated from artificial water bodies located in Poland," Acta Protozoologica, vol. 54, 2015.
M. Rezaeian, M. Niati, S. Farnia, and H. A. MOTEVALI, "Isolation of Acanthamoeba spp. from different environmental sources," 2008.
M. Rezaian, F. Bagheri, S. Farnia, and Z. Babai, "Isolation of pathogenic amoebae (Naegleria and Acanthamoeba) from water sources and margin soils of rivers and lakes in Kazerun," 2002.
K. A. Coşkun, S. Özçelik, L. Tutar, N. Elaldı, and Y. Tutar, "Isolation and identification of free-living amoebae from tap water in Sivas, Turkey," BioMed research international, vol. 2013, 2013.
M. Eftekhar, A. M. E. NAZEM, A. HAGHIGHI, K. SHARIFI, Z. Nouchi, and A. Athari, "Detection of Acanthamoeba from fresh water using polymerase chain reaction," 2009.
B. Armand, M. Motazedian, and Q. Asgari, "Isolation and identification of pathogenic free-living amoeba from surface and tap water of Shiraz City using morphological and molecular methods," Parasitology research, vol. 115, pp. 63-68, 2016.
V. Thomas, J. F. Loret, M. Jousset, and G. Greub, "Biodiversity of amoebae and amoebae‐resisting bacteria in a drinking water treatment plant," Environmental microbiology, vol. 10, pp. 2728-2745, 2008.
A. J. Martinez and G. S. Visvesvara, "Free‐living, amphizoic and opportunistic amebas," Brain Pathology, vol. 7, pp. 583-598, 1997.
A. Magnet, S. Fenoy, A. Galván, F. Izquierdo, C. Rueda, C. F. Vadillo, et al., "A year long study of the presence of free living amoeba in Spain," Water research, vol. 47, pp. 6966-6972, 2013.
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