Detection of Cryptosporidium parvum by modified acid-fast stain among cancer patients in Thi-Qar province
DOI:
https://doi.org/10.32792/utq/utjsci/v10i2.1062Keywords:
Cryptosporidiosis, acid-fast stain, Thi-Qar province, cryptosporidium parvumAbstract
Cryptosporidium parvum is a widespread opportunistic parasite belonging to the family Apicomplexa that completes its life cycle in a single host and is found frequently in immunocompromised persons, especially in patients with cancer. This research's objectives were to determine the prevalence of Cryptosporidium parvum infection among cancer patients in the Thi-Qar Province of Iraq, using modified Ziehl-Neelsen (MZN) stain. This study included 300 stool samples, 200 of which were for patients with cancer and 100 were for healthy people (the control group). Based on the modified acid-fast stain, the results showed the rate of infection with Cryptosporidium parvum among cancer patients was 54.0% (108/200) compared to 30.0% (30/100) in the control group, and there were statistically significant differences between them .And the percentage of infection among cancer patients in males was higher than in females, but there were no significant differences between them, as the percentage was 57.14% and 52.03%, respectively. As for the age groups Cryptosporidium parvum it affects all age groups in different proportions. The study showed that the highest infection rate among the age groups reached ?60 years at a rate of 64.44%, and the lowest infection rate among the age group ?20 years at a rate of 35.71%. As for the number doses of chemotherapy the results showed that the infection rate was higher in patients who received half and more than half of the doses of chemotherapy, where the percentage of those who received 16-20 doses (64.0%)
Received: 2023-06-15
Revised : 2023-07-03
Accepted: 2023-07-05
References
S. Z. Al-Rumaidh, B. A. AL-Aboody, and A. Salman Abdul-Al hasan, “Investigation of infection of intestinal parasites Entamoeba histolytica and Giardia lamblia among patients which attending of the Health Centers of Gharraf City / Thi- Qar province”, jsci, vol. 6, no. 3, pp. 25–29, Apr. 2019.
D. Costa et al., “Epidemiology of Cryptosporidiosis in France from 2017 to 2019,” Microorganisms, vol. 8, no. 9, p. 1358, Sep. 2020, doi: 10.3390/microorganisms8091358.
U. Ryan, A. Zahedi, Y. Feng, and L. Xiao, “An Update on Zoonotic Cryptosporidium Species and Genotypes in Humans,” Animals, vol. 11, no. 11, p. 3307, Nov. 2021, doi: 10.3390/ani11113307.
I. A. Khalil,C. Troeger, P. C. Rao, B. F. Blacker, A. Brown, T. G. Brewer,et al ," Morbidity, mortality, and long-term consequences associated with diarrhea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study" Global Health,vol. 6,no.7,pp. 758-768, July 2018
U. Ryan, N. Hijjawi, and L. Xiao,'' Foodborne Cryptosporidiosis'' Journal of Parasitology, vol.48,no.1,pp .1-12 ,Jan 2018.
E. Butkeviciute, H.J. Prudden, M. Jit,et al.,'' Global diarrhoea-associated mortality estimates and models in children: recommendations for dataset and study selection'' Vaccine,vol.39,no.32,pp. 4391–4398. July 2021.
R. Razakandrainibe, E. Hadji, I. Diawara, D. Costa, and G. Gargala,'' Common occurrence of Cryptosporidium in asymptomatic and symptomatic calves in France'' PLoS neglected tropical diseases,vol.12, no .3,p . 0006355, 29 Mar. 2018, doi:10.1371/journal.pntd.0006355
A. Sateriale, et al. “A Genetically Tractable, Natural Mouse Model of Cryptosporidiosis Offers Insights into Host Protective Immunity.” Cell host & microbe, vol. 26, no.1, pp. 135-146.Jul.2019, doi:10.1016/j.chom.2019.05.006
N. Holubová, et al. “Description of Cryptosporidium ornithophilus n. sp. (Apicomplexa: Cryptosporidiidae) in farmed ostriches.” Parasites & vectors, vol. 13, no.1, pp. 340. 8 Jul. 2020, doi:10.1186/s13071-020-04191-2
A. H.Awwad, and S. K. Nasser, “Study of the prevalence of Cryptosporidium sp. cysts in some water sources of Basra Governorate” Dhi Qar Science Journal,vol. 4 , no .3 , pp.9-12 ,June .2014 https://search.emarefa.net/detail/BIM-599956
M. K.Oliewi, and A. K. Al-Hamairy, “Epidemiological and Diagnostic Study for Diarrheic Parasites (Entamoeba histolytica, Giardia lamblia, and Cryptosporidium sp.) Among Diarrheic Infected Patients by Using Multiplex” Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol.7, no.1, pp. 438–447, Jan.2016
R. S. Akram, “Molecular detection and prevalence of Cryptosporidium parvum, Entamoeba histolytica, and Giardia lamblia among patients with diarrhea at Al-Rifea city” Journal of Global Pharma Technology, vol .18 ,no .1 ,pp .18-27,Nov .2018
T. Hailegebriel, “Prevalence of intestinal parasitic infections and associated risk factors among students at Dona Berber primary school, Bahir Dar, Ethiopia.” BMC infectious diseases, vol. 17, no.1, pp. 362. May. 2017, doi: 10.1186/s12879-017-2466-x
K. A. Nasir, A. A. Hama , and S. I. Ali, "Prevalence of Cryptosporidiosis among cancer patients in Sulaimani province/Iraq." Int J Psychosoc Rehabil, vol.24, no.09, Jul .2020
R. Berahmat, et al. “Cryptosporidium infection in children with cancer undergoing chemotherapy: how important is the prevention of opportunistic parasitic infections in patients with malignancies?” Parasitology research vol. 116, no .9, pp 2507-2515.Sep .2017, doi: 10.1007/s00436-017-5560-5
D. Burgner, et al. “Epidemiology of Cryptosporidium parvum in symptomatic paediatric oncology patients.” Journal of paediatrics and child health, vol. 35, no.3, pp. 300-2, Jun. 1999, doi:10.1046/j.1440-1754.1999.00361.x
R. Rivera-Luna, et al. "Childhood acute leukemia and intestinal parasitosis." Leukemia ,vol.3,no.11 , pp 825-826. No. 1989
M.K. Oleiwi, '' prevalence study for main protozoa diarrheal among patients by using microscopically and molecular methods in Babylon province'' College of Science for Women, Babylon University, pp.23- 25.2015
M. M.Yacoub,'' Intestinal Protozoa and Cryptosporidium genotypes in North of West Bank/Palestine'' Doctoral dissertation, 2014
[[20 F. I. Mohammad,. "Detecting of virulence factors COWP gene and CP15 gene for Cryptosporidium parvum by polymerase chain reaction (PCR)." Al-Qadisiyah Journal of Pure Science , vol .23, no.2, pp. 39-47,Jul.2018
H. Saneian, et al. “Infection Rate of Cryptosporidium parvum among Diarrheic Children in Isfahan.” Iranian journal of pediatrics vol. 20, no.3, pp. 343-7, Sep. 2010
A. K. Al–Mamouri,'' Epidemiology of intestinal parasites and head lice in pupils of some primary schools at Al- Mahaweel district, Babylon province'' Science. Collage., BabylonUniv, pp.122,2000
A. R. Monsef,'' Frequency of intestinal parasites in patients with malignancy, admitted in oncology ward of Sina Hospital, Hamadan'' J Gorgan Univ Med Sci, vol.9.no. 4, pp .51-55, Jun .2008
A. Barazesh, et al. “The prevalence of intestinal parasites in hemodialysis patients in Bushehr, Iran.” Hemodialysis international. International Symposium on Home Hemodialysis vol. 19, no.3, pp. 447-51.Jul 2015, doi:10.1111/hdi.12272
U. Aksoy, et al. “Intestinal parasites in children with neoplasms.” The Turkish journal of pediatrics vol. 45, no .2, pp.129-32, Jun.2003
A.Mathew,et al '' Infection rate of Cryptosporidium parvum among diarrhoea children in Ibadan, Oyo State, Nigeria'' SJAMS, vol.2 ,no .6 ,pp . 3127-3131, Jul .2014
M. A, Mohaghegh, et al. “Prevalence and clinical features of Cryptosporidium infection in hemodialysis patients.” Gastroenterology and hepatology from bed to bench vol. 10, no.2 , pp. 137-142 , Spring. 2017
M.R, Mahmoudi, et al. “Intestinal protozoan and helminthic infections among hemodialysis and cancer patients.” Parasitology research vol. 119, no .9, pp. 3053-3059. Sep. 2020, doi: 10.1007/s00436-020-06774-5
S. Chandramathi, et al. “Infections of Blastocystis hominis and microsporidia in cancer patients: are they opportunistic?” Transactions of the Royal Society of Tropical Medicine and Hygiene vol. 106, no .4 pp. 267-9, Apr.2012. doi:10.1016/j.trstmh.2011.12.008
E.F, Solomayer, et al. “Influence of adjuvant hormone therapy and chemotherapy on the immune system analysed in the bone marrow of patients with breast cancer.” Clinical cancer research : an official journal of the American Association for Cancer Research vol. 9,no.1, pp. 174-80 , Jan. 2003
R. Koivusalo, and H .Sakari, “The cytotoxicity of chemotherapy drugs varies in cervical cancer cells depending on the p53 status.” Cancer biology & therapy vol. 3, no.11, pp. 1177-83, Nov .2004, doi:10.4161/cbt.3.11.1340
M Al-Mukhtar, Abdul-Salam, Nawfal Y Al-Dabbagh, and Nihad S Al-Sherrefat. " Microsporidiosis in malignancy affected patients in Mosul, Iraq " The Medical Journal of Basrah University , vol.26 ,no.1 ,pp 28-31.Jan. 2008
M.M, Sanad, et al. "Opportunistic coccidian parasites among Saudi cancer patients presenting with diarrhea: prevalence and immune status." Research Journal of Parasitology , vol.9 ,no.2, pp. 55-63, Jun .2014
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