A Human CCL3L1 gene expression in blood donors infected with HIV-1
DOI:
https://doi.org/10.32792/utq/utjsci/v10i1.1060Keywords:
CCL3L1, β- actin , gene expression, HIV-1Abstract
The CCL3L1 gene plays an important role in regulating immunity against many pathogens. The aim of this study, which was conducted in Thi-Qar province from February 2022 to February 2023, was to estimate the gene expression of the CCL3L1 gene in people infected with HIV compared to the control group. There was an increase in the level of gene expression in patients, where it was (17.02778 ) compared to the control group, which was (1.428108) using qPCR. There is a need for more molecular studies and follow-up of genetic and immunological changes in infected patients.
References
-Adewoye, A. B., Shrine, N., Odenthal-Hesse, L., Welsh, S., Malarstig, A., Jelinsky, S., ... & Wain, L. V. (2018). Human CCL3L1 copy number variation, gene expression, and the role of the CCL3L1-CCR5 axis in lung function. Wellcome Open Research, 3.?
- Ali, A. K. (2014). X-Ray Repair Cross Complementing 4 (XRCC4) Gene Expressions as Biomarkers for Detection of Ionizing Radiation Exposure. University of Thi-Qar Journal of Science, 4(2), 23-30.?
-Almal, S. H., & Padh, H. (2012). Implications of gene copy-number variation in health and diseases. Journal of human genetics, 57(1), 6-13.?
-Arenzana-Seisdedos, F., & Parmentier, M. (2006). Genetics of resistance to HIV infection: Role of co-receptors and co-receptor ligands. In Seminars in immunology (Vol. 18, No. 6, pp. 387-403). Academic Press.
-Bhattacharya, T., Stanton, J., Kim, E. Y., Kunstman, K. J., Phair, J. P., Jacobson, L. P., & Wolinsky, S. M. (2009). Ccl3l1 and hiv/aids susceptibility. Nature medicine, 15(10), 1112-1115.
-Gonzalez, E., Kulkarni, H., Bolivar, H., Mangano, A., Sanchez, R., Catano, G., ... & Ahuja, S. K. (2005). The influence of CCL3L1 gene-containing segmental duplications on HIV-1/AIDS susceptibility. Science, 307(5714), 1434-1440.?
-Hawe, J. S., Wilson, R., Schmid, K. T., Zhou, L., Lakshmanan, L. N., Lehne, B. C., ... & Chambers, J. C. (2022). Genetic variation influencing DNA methylation provides insights into molecular mechanisms regulating genomic function. Nature genetics, 54(1), 18-29.
-Huik, K., Sadam, M., Karki, T., Avi, R., Krispin, T., Paap, P., ... & Lutsar, I. (2010). CCL3L1 copy number is a strong genetic determinant of HIV seropositivity in Caucasian intravenous drug users. The Journal of infectious diseases, 201(5), 730-739.?
-Hussain, Y. A., Mohammed, K. A., & Ali, N. H. (2023). Studying the estimation levels of inflammatory markers and NF-? B in MDR and DS-TB patients. University of Thi-Qar Journal of Science, 10(1 (SI)).?
-Isa, I. I. M., Jamaluddin, J., Achim, N. H., & Abubakar, S. (2020). Population-specific profiling of CCL3L1 copy number of the three major ethnic groups in Malaysia and the implication on HIV susceptibility. Gene, 754, 144821.
-Jamaluddin, J., Mohd Khair, N. K., Vinodamaney, S. D., Othman, Z., & Abubakar, S. (2020). Copy number variation of CCL3L1 among three major ethnic groups in Malaysia. BMC genetics, 21(1), 1-8.?
-Kaslow, R. A., Dorak, T., & Tang, J. (2005). Influence of host genetic variation on susceptibility to HIV type 1 infection. The Journal of infectious diseases, 191(Supplement_1), S68-S77.
-Kuhn, L., Schramm, D. B., Donninger, S., Meddows-Taylor, S., Coovadia, A. H., Sherman, G. G., ... & Tiemessen, C. T. (2007). African infants’ CCL3 gene copies influence perinatal HIV transmission in the absence of maternal nevirapine. AIDS (London, England), 21(13), 1753.?
-Li, J., Liu, Y., Kim, T., Min, R., & Zhang, Z. (2010). Gene expression variability within and between human populations and implications toward disease susceptibility. PLoS computational biology, 6(8), e1000910.
-Liu, R., Paxton, W. A., Choe, S., Ceradini, D., Martin, S. R., Horuk, R., ... & Landau, N. R. (1996). Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV-1 infection. Cell, 86(3), 367-377.
-Mori, R., Wang, Q., Danenberg, K. D., Pinski, J. K., & Danenberg, P. V. (2008). Both ??actin and GAPDH are useful reference genes for normalization of quantitative RT?PCR in human FFPE tissue samples of prostate cancer. The Prostate, 68(14), 1555-1560.?
-Nakajima, T., Kaur, G., Mehra, N., & Kimura, A. (2008). HIV-1/AIDS susceptibility and copy number variation in CCL3L1, a gene encoding a natural ligand for HIV-1 co-receptor CCR5. Cytogenetic and genome research, 123(1-4), 156-160
-Pilotti, E., Elviri, L., Vicenzi, E., Bertazzoni, U., Re, M. C., Allibardi, S., ... & Casoli, C. (2007). Postgenomic up-regulation of CCL3L1 expression in HTLV-2–infected persons curtails HIV-1 replication. Blood, 109(5), 1850-1856.
-Urban, T. J., Weintrob, A. C., Fellay, J., Colombo, S., Shianna, K. V., Gumbs, C., ... & Goldstein, D. B. (2009). Ccl3l1 and hiv/aids susceptibility. Nature medicine, 15(10), 1110-1112.
-Walker, W. E., Kurscheid, S., Joshi, S., Lopez, C. A., Goh, G., Choi, M., ... & Fikrig, E. (2015). Increased levels of macrophage inflammatory proteins result in resistance to R5-tropic HIV-1 in a subset of elite controllers. Journal of virology, 89(10), 5502-5514.?
-Weinberger, L. S., Burnett, J. C., Toettcher, J. E., Arkin, A. P., & Schaffer, D. V. (2005). Stochastic gene expression in a lentiviral positive-feedback loop: HIV-1 Tat fluctuations drive phenotypic diversity. Cell, 122(2), 169-182.
Downloads
Published
License
Copyright (c) 2023 University of Thi-Qar Journal of Science
This work is licensed under a Creative Commons Attribution 4.0 International License.
