Molecular Detection of Babesia bovis Isolated from Cows in Thi-Qar Province
DOI:
https://doi.org/10.32792/utq/utjsci/v12i2.1476Abstract
The present study was designed to determine the bovar2A gene in Babesia bovis Thus gene encodes a variant erythrocyte surface antigen that is unique to B. bovis was detected in the blood of infected cows in Thi-Qar province during the period of February to June 2019. A total of 96 blood samples were collected from the cows of both sexes. The Polymerase Chain Reaction (PCR)is used to detect the bovar2A gene in Babesial infection status in cows. The PCR was used to distinguish Babesia bovis with a high level of sensitivity, capable to detection every species of B. bovis, and no evidence of non-specific amplification. The PCR results showed the bovar2A gene was harbored in 64 of 96 samples (66.66%) of Babesia bovis. The current study concluded that the bovar2A gene is useful for diagnosing B. bovis and the rising incidence of the disease in southern Iraq.
Received: 2025-09-17
Revised: 2025-11-18
Accepted: 2025-12-09
References
[1] R. Y.E. Abdo-Sakaya,"Some studies on diagnosis on babesiosis. M. Sc. thesis, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
[2] T.I. Al-Alousi, I.K. Zangana, and M.Sh. Rhayman, "Occurrence of babesiosis in gazelles in Mosul. Iraq". J. Scien.6(3):76,1988.
[3] K.A.H. Ameen, B.A. Abdullah, and R.A. Abdul-Razaq, "Sero-prevalence of Babesia bigemina and Anaplasma marginale in domestic animals in Erbil, Iraq". Iraq. J Vet Sci; 26: 109-114, 2012.
[4] L. Ferreri, D. Benitez, Dominguez, M. Rodriguez, A.; Asenzo, G. ; M. Mesplet, M. "Florin- Christensen, and, L. Schnittger, "Water buffalos as carriers of Babesia bovis in Argentina". Animal Biodiversity and Emerging Diseases: Ann. N.Y. Acad. Sci. 1149:149-151. 2008.
[5] K.T Friedhoff and R. D. Smith, "Transmission of babesia by ticks," in Restic ,M and Carrier J.P (edds) Academic press. San Diego pp 267 - 321.1981.
[6] W.L Goof, W.C. Jhonson, W. Teo, R.A. Valdez, and S.M. Parish, "Age related innate immune response in calves in Babesia bovis involve IL2 induction and IL10 modulation," Ann N.Y .Acad .Sci. 969: 164 - 168. 2002.
[7] L. He, , H.H. Feng, W.J. Zhang, Q.L. Zhang, R. Fang, L.X. Wang, Р. Tu, Y.Q. Zhou, J.L. Zhao, and M.C. Oosthuizen, "Occurrence of Theileria and Babesia species in water buffalo (Bubalus babalis, Linnaeus, (1758) in the Hubei province, South China, " J. Vet Parasitol, 186, 490-496,2012.
[8] T.C.G. Oliveira-Sequeira, M.C.S. Oliveira, J. Araújo, A.F.T. Amarante, and H.N Oliveira, "PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle, " Int. J. Parasitol. 35, 105-111, 2005.
[9] K. H. Sabbara, and N. A. Aaiz, "Molecular detection of Babesia bovis and Babesia bigemina in Cattle in Al-Qadisiyah Province," The Iraqi Journal of Veterinary Medicine.;40(2):155-158, 2016.
[10] D.E. Ozlem, and F. Sevinc, " Seroepidemiology study of B. bigemina in cattle in the Konya province, Turkey," Bull Vet Inst Pulawy; 53: 645-649, 2009.
[11] S. Rajabi, B. E. Nejad, &M. Tavassoli, "A molecular study of Babesia spp in West-Azerbaijan province – Iran, " Vet. Res., 8 (4) : 299 – 306, 2017.
[12] F. Saad, M. Khaisroom, K. , Khan, and N. Akbar, "Prevalence and molecular detection of babesia in the slaughter animals of Peshawar pakhunkhawa Pakistan, " International J. Microbio,4 (8) : 1030 - 1036 ,2017.
[13] K. Sabar, and N.N. Aaiz, "Molecular detection of Babesia bovis and Babesia bigemina in cattle in Al Qadissiya province, " raqi J. Vet. Med. 5 (8) : 330 – 336, 2015.
[14] M.A. Terkawi, H. Alhasan, and N. X. Huyen, "Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria, " Vet Parasitol; 187: 307-311, 2012.
[15] M. A. Terkawi, N.X. Huyen, C. Shinuo, T.; Maklon, K. Inpankaew, M. Aboulaila, A. Ueno, Y. K. N. Yokoyama, S. Jittapalapong, X. Xuan, and I. Igarashi, "Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand". Vet. Parasitol, 178, 201-207.
[16] T.R.A. El-Naga, S. Barghash, "Blood parasites in camels (Camelus dromedarius) in northern west coast of Egypt, " J Bacteriol Parasitol. 2016;7(1):1-8. DOI: 10.4172/2155-9597.1000258
[17] M.C.S. Oliveira,T,C.G. Oliveira-Sequeira, J.P. Araujo, A.F.T. Amarante, and, H.N. Oliveira, "Babesia spp. infection in Boophilus microplus engorged females and eggs in São Paulo State, Brazil, " Vet Parasitol.130(1–2):61–7, 2005.
[18] F. Adham, E. Abd-El-Samie, R. Gabre, and, H. El Hussein "Detection of tick blood parasites in Egypt using PCR assay I-Babesia bovis and Babesia bigemina, " Parasitol Res.;105(3):721–30, 2009.
[19] A.A. AL-Hosary, "Comparison between Conventional and Mo- lecular Methods for Detection of Bovine Babesiosis (Babesia bovis infection) in Tick Infested Cattle in Upper Egypt, " J Parasit Dis. 2016;1–4.
[20] A. Sharma, L. Singla , A. Tuli , P. Kaur, B. Batth, and M. Javed, "Molecular prevalence of babesia bigemina and trypanosoma evansi in dairy animals from Punjab, India, by duplex PCR: A step forward to the detection and management of concurrent latent infections, " Biomed Res Int. 1, 8. 2013.
[21] D. H. Sawitri, A. H. Wardhana, F. Ekawasti, and D. A. Dewi, "Parasitological and Molecular Detection of Babesiosis in Cattle and Buffalo in West and Central Java, " Advances in Biological Sciences Research, 18: 135-140, 2022.
[17] M.C.S. Oliveira,T,C.G. Oliveira-Sequeira, J.P. Araujo, A.F.T. Amarante, and H.N. Oliveira, "Babesia spp. infection in Boophilus microplus engorged females and eggs in São Paulo State, Brazil, " Vet Parasitol.130(1–2):61–7, 2005.
[18] F. Adham, E. Abd-El-Samie, R. Gabre, and H. El Hussein "Detection of tick blood parasites in Egypt using PCR assay I-Babesia bovis and Babesia bigemina, " Parasitol Res.;105(3):721–30, 2009.
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