Microbial Detection and Antibiotic  Susceptibility patterns  of Clinical Isolates from Women With Urinary Tract Infection in AL-Nasiriyah City/Iraq


  • Muna Jihad College of Science/ University Thi_Qar
  • Manal Salih College of Science , University of Thi-Qar




Microbial infection, Antibiotic Resistance, UTI in women, MDR


Urinary tract infections (UTIs) in women are one of the most common diseases affecting at different ages of the life. This study was aimed to investigate the uropathogenes in women with UTI and to test their antibiotic susceptibility pattern. A total of 150 urine samples were collected from patients women and healthy in Thi-Qar Province, southern Iraq, between December 2022 and March 2023.Urine samples were collected from each participant women and cultured on media, then identified by different laboratory methods such as characteristic of colony on culture media, Gram stain, biochemical tests and morphologic chromatic properties on Hi UTI chromogenic agar and confirmed by API 20E system. The majority of females (44%) were the age groups 25 to 34 years followed by the (34%) patients were age group from 15 to 24 years. Antibiotic susceptibility testing for bacteria species was performed using the Kirby,Bauer disc, diffusion technique. Out of 136 different isolates with noticeable growth were found in the urine sample after microbiological cultures were performed, bacteria species was represented (63.24%) more than Candida species which was (36.76%). The most frequent bacterium was Staphylococcus species (40.69%) followed by Escherichia coli (18.60%). These bacterial exhibited extremely resistance to antibiotics. Gram positive isolates showed the highest resistant against Amoxicillin-clavulanate (100%), Ampicillin (100%), Oxacillin (100%), and Trimethoprim (91.66%), on the other hand Gram negative bacteria also showed the highest resistance against Amoxicillin-clavulanate (100%), Piperacillin (100 %), and  both Ceftazidime and Nitrofurantoin (86.36 %). Other antibiotics showed different results by different bacterial species.


M. Mirzarazi, S. E. Rezatofighi, M. Pourmahdi, and M. R. Mohajeri, “Antibiotic resistance of isolated gram negative bacteria from urinary tract infections (UTIs) in Isfahan,” Jundishapur J. Microbiol., vol. 6, no. 8, pp. 6–10, 2013, doi: 10.5812/jjm.6883.

G. Liu, L. E. Thomsen, and J. E. Olsen, “Antimicrobial-induced horizontal transfer of antimicrobial resistance genes in bacteria: A mini-review,” J. Antimicrob. Chemother., vol. 77, no. 3, pp. 556–567, 2022, doi: 10.1093/jac/dkab450.

M. A. K. Al-saadi, N. M. S. Al-hilli, and N. A. Sheriff, “Association of TLR4 Polymorphisms with Susceptibility to Urinary Tract Infection Caused by Gram Positive Bacteria in Pregnant Women,” Indian J. Public Heal. Res. Dev., vol. 11, no. 6, 2020, doi: 10.37506/ijphrd.v11i4.9201.

F. R. DeLeo, B. A. Diep, and M. Otto, “Host Defense and Pathogenesis in Staphylococcus aureus Infections,” Infect. Dis. Clin. North Am., vol. 23, no. 1, pp. 17–34, 2009, doi: 10.1016/j.idc.2008.10.003.

C. Elias, L. Moja, D. Mertz, M. Loeb, G. Forte, and N. Magrini, “Guideline recommendations and antimicrobial resistance: The need for a change,” BMJ Open, vol. 7, no. 7, pp. 1–8, 2017, doi: 10.1136/bmjopen-2017-016264.

S. Selim et al., “Incidence of Vancomycin-Resistant Staphylococcus aureus Strains among Patients with Urinary Tract Infections,” Antibiotics, vol. 11, no. 3, pp. 1–8, 2022, doi: 10.3390/antibiotics11030408.

F. S. Pasillas Fabian, R. Cremades, E. Sandoval Pinto, C. Beas Ruiz Velasco, C. J. Hernandez Rios, and E. Sierra-Diaz, “Microbiological profile of urinary tract infections in a tertiary medical facility in Western Mexico: An update,” Sci. Prog., vol. 104, no. 1, pp. 1–13, 2021, doi: 10.1177/00368504211000886.

H. Mousa and T. Abd Al-Amir, “Infections in Urinary Tract among Pregnant Women in Nasiriya City, Iraq,” Univ. Thi-Qar J. Sci., vol. 10, no. 1, pp. 117–121, 2023, doi: 10.32792/utq/utjsci/v10i1.1039.

P. Ramart and A. L. Ackerman, “Recurrent Urinary Tract Infection in Women from a Urologist’s Perspective,” Siriraj Med. J., vol. 75, no. 1, pp. 55–61, 2023, doi: 10.33192/smj.v75i1.260531.

A. Ullah, “Prevalence of symptomatic urinary tract infections and antimicrobial susceptibility patterns of isolated uropathogens in kohat region of Pakistan,” MOJ Biol. Med., vol. 3, no. 2, 2018, doi: 10.15406/mojbm.2018.03.00082.

A. A. Al-Naqshbandi, M. A. Chawsheen, and H. H. Abdulqader, “Prevalence and antimicrobial susceptibility of bacterial pathogens isolated from urine specimens received in rizgary hospital — Erbil,” J. Infect. Public Health, vol. 12, no. 3, pp. 330–336, 2019, doi: 10.1016/j.jiph.2018.11.005.

B. Kot, “Antibiotic Resistance among Uropathogenic Escherichia coli,” Polish J. Microbiol., vol. 68, no. 4, pp. 403–415, 2019, doi: 10.33073/PJM-2019-048.

S. ISA, K. MUSTAPHA Y, J. HAMZA A, and S. RASHIDA, “Evaluation of the Incidence of Uropathogenic Bacteria Among Pregnant Women Attending Antenatal Clinic Doma Hospital Gombe,” Bima J. Sci. Technol., vol. 6, no. 03, pp. 129–135, 2022, doi: 10.56892/bima.v6i03.60.

Muna F. Jahad and Mohammed Hashim AL-Yasiri, “Microbial Infections Associated with Diabetic Foot Ulcer in Nasiriyah City,” Indian J. Forensic Med. Toxicol., vol. 15, no. 2, pp. 2887–2892, 2021, doi: 10.37506/ijfmt.v15i2.14808.

I. Khatoon, S. Khanam, A. Azam, S. Qadeer, S. Naz, and N. U. Hassan, “Incidence Pattern, Antibiotic Susceptibility Pattern and Associated Risk Factors of Bacterial Uropathogens Among General Population of Pakistan,” Infect. Drug Resist., vol. 16, no. 6, pp. 4995–5005, 2023, doi: 10.2147/IDR.S418045.

B. S. Fazly Bazzaz, S. Darvishi Fork, R. Ahmadi, and B. Khameneh, “Deep insights into urinary tract infections and effective natural remedies,” African J. Urol., vol. 27, no. 1, pp. 1–13, 2021, doi: 10.1186/s12301-020-00111-z.

B. John et al., “Occurrence of Staphylococcus associated with Urinary Tract Infections among women attending Ibrahim Badamasi Babangida (IBB) specialist hospital, Minna, Nigeria.,” Tanzan. J. Health Res., vol. 24, no. 2, pp. 17–30, 2023, doi: 10.4314/thrb.v24i2.2.

E. N. AKYOL ONDER and P. ERTAN, “Antibiotic Resistance Patterns in Pediatric Urinary Tract Infections: A Comparison Between 2013-2015 and 2019-2021,” Celal Bayar Üniversitesi Sağlık Bilim. Enstitüsü Derg., vol. 9, no. 3, pp. 381–385, 2022, doi: 10.34087/cbusbed.1076551.

G. Shaheen et al., “Therapeutic potential of medicinal plants for the management of urinary tract infection: A systematic review,” Clin. Exp. Pharmacol. Physiol., vol. 46, no. 7, pp. 613–624, 2019, doi: 10.1111/1440-1681.13092.

R. D. Klein and S. J. Hultgren, “Urinary tract infections: microbial pathogenesis, host–pathogen interactions and new treatment strategies,” Nat. Rev. Microbiol., vol. 18, no. 4, pp. 211–226, 2020, doi: 10.1038/s41579-020-0324-0.

S. Sahel and I. Abid, “Phenotypic detection of extended spectrum beta-lactamases (ESBLs) in Gram-negative bacilli isolated from deferent clinical source,” Univ. Thi-Qar J. Sci., vol. 9, no. 2, pp. 46–49, 2022, doi: 10.32792/utq/utjsci/v9i2.902.

G. D. Christensen et al., “crossm CLSI Methods Development and Standardization Working,” J. Clin. Microbiol., vol. 56, no. 4, pp. 1–10, 2018.

Ö. Kandemir, A. Akdaǧ, and A. Öne Kurt, “Antibiotic susceptibility of bacterial strains isolated from patients with community acquired urinary tract infections in mersin,” TAF Prev. Med. Bull., vol. 11, no. 5, pp. 589–598, 2012, doi: 10.5455/pmb.1-1321538566.

M. Shirvani, A. Keramati, and M. Esmaeli, “Evaluating the pattern of antibiotic resistance of urinary tract infection (UTI)-causing bacteria in the urine culture samples of patients in the infectious ward of Imam Khomeini Hospital, Kermanshah, in Iran from 2016–2018,” African J. Urol., vol. 29, no. 1, pp. 1–11, 2023, doi: 10.1186/s12301-023-00364-4.

M. A. Mohammed, T. M. S. Alnour, O. M. Shakurfo, and M. M. Aburass, “Prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in Messalata Central Hospital, Libya,” Asian Pac. J. Trop. Med., vol. 9, no. 8, pp. 771–776, 2016, doi: 10.1016/j.apjtm.2016.06.011.

H. A. Salih and A. T. Abbas, “Microorganism Prevalence and Antibiotic Sensitivity for Imipenem and Meropenem in Patients with Diabetic Foot Ulcers in Thi Qar, Iraq,” J. Pharm. Negat. Results, vol. 13, no. S01, pp. 1047–1051, 2022, doi: 10.47750/pnr.2022.13.s01.125.

G. E. A. Dogonchi AA Ardebili A Yazdansetad S and P. A, “Metallo‑β‑lactamase‑mediated resistance among clinical carbapenem‑resistant,” Tzu Chi Med. j, vol. 30, no. 2, pp. 90–96, 2018, doi: 10.4103/tcmj.tcmj.

H. Jassim, “The Prevalence of blaCTX-M, blaTEM Genes in Bacteria Isolated From Bladder Cancer Patients with Urinary Tract Infections,” Univ. Thi-Qar J. Sci., vol. 10, no. 1, pp. 16–26, 2023, doi: 10.32792/utq/utjsci/v10i1.919.

F. M. Al-Asady, D. A. Al-Saray, and A. E. Al-Araji, “Screening of urinary tract bacterial infections and their antibiogram among non-pregnant women admitted to Al-Sadiq hospital, Iraq,” AIP Conf. Proc., vol. 2386, no. 1, pp. 1–9, 2022, doi: 10.1063/5.0066884.

A. L. Flores-Mireles, J. N. Walker, M. Caparon, and S. J. Hultgren, “Urinary tract infections: Epidemiology, mechanisms of infection and treatment options,” Nat. Rev. Microbiol., vol. 13, no. 5, pp. 269–284, 2015, doi: 10.1038/nrmicro3432.

P. Behzadi, E. Urbán, M. Matuz, R. Benkő, and M. Gajdács, “The Role of Gram-Negative Bacteria in Urinary Tract Infections: Current Concepts and Therapeutic Options,” Adv. Exp. Med. Biol., vol. 1323, no. 6, pp. 35–69, 2021, doi: 10.1007/5584_2020_566.

G. Mancuso, A. Midiri, E. Gerace, M. Marra, S. Zummo, and C. Biondo, “Urinary Tract Infections: The Current Scenario and Future Prospects,” Pathogens, vol. 12, no. 4, pp. 1–17, 2023, doi: 10.3390/pathogens12040623.

M. Negri, R. dos S. B. R. W. Bárbara Martins Lima, R. A. S. Molina, L. C. de S. Carla Maria, Germano, Débora Gusmão Melo, Avó, and L. R. da S. De, “Prevalence and antimicrobial resistance profile of pathogens isolated from patients with urine tract infections admitted to a university hospital in a medium-sized Brazilian city,” Rev. Inst. Med. Trop. Sao Paulo, vol. 4, no. 1, pp. 1–7, 2010, [Online]. Available: http://kiss.kstudy.com/journal/thesis_name.asp?tname=kiss2002&key=3183676

Y. Alhamadani and A. Oudah, “Study of the Bacterial Sensitivity to different Antibiotics which are isolated from patients with UTI using Kirby-Bauer Method,” J. Biomed. Biochem., vol. 1, no. 2, pp. 1–5, 2022, doi: 10.57238/jbb.2022.19387.

A. Halinski et al., “Spectrum of Bacterial Pathogens from Urinary Infections Associated with Struvite and Metabolic Stones,” Diagnostics, vol. 13, no. 1, pp. 1–10, 2023, doi: 10.3390/diagnostics13010080.

M. Drahansky et al., “Estimation of Some Plant Extract Activity against Bacterial Cystitis Isolated from Urinary Tract Infection,” in Intech, no. 2, 2016, p. 13. doi: http://dx.doi.org/10.5772/57353.

A. Bitew, T. Molalign, and M. Chanie, “Species distribution and antibiotic susceptibility profile of bacterial uropathogens among patients complaining urinary tract infections,” BMC Infect. Dis., vol. 17, no. 1, pp. 1–8, 2017, doi: 10.1186/s12879-017-2743-8.

S. A. Ali, S. Mandal, A. Georgalas, and S. A. D. Gilani, “A Pattern of Antibiotic Resistance in Gram-Negative Rods Causing Urinary Tract Infection in Adults,” Cureus, vol. 13, no. 1, pp. 1–6, 2021, doi: 10.7759/cureus.12977.

W. D. Seifu and A. D. Gebissa, “Prevalence and antibiotic susceptibility of Uropathogens from cases of urinary tract infections (UTI) in Shashemene referral hospital, Ethiopia,” BMC Infect. Dis., vol. 18, no. 1, pp. 1–9, 2018, doi: 10.1186/s12879-017-2911-x.

S. Choubey and M. Verma, “Common cause of UTI and its regimen a review paper,” J. Adv. Microbiol. Res. 2022;, vol. 3, no. 2, pp. 83–86, 2022.

Q. hassan Aubais aljelehawy, L. H. Hadi Alshaibah, and Z. K. Abbas Al- Khafaji, “Evaluation of virulence factors among Staphylococcus aureus strains isolated from patients with urinary tract infection in Al-Najaf Al-Ashraf teaching hospital,” Cell. Mol. Biomed. Reports, vol. 1, no. 2, pp. 78–87, 2021, doi: 10.55705/cmbr.2021.144995.1017.

T. E., T. M., M. Y., K. B., and S. T., “Asymptomatic urinary tract infection among pregnant women attending the antenatal clinic of Hawassa Referral Hospital, Southern Ethiopia.,” BMC Res. Notes, vol. 7, p. 155, 2014, [Online]. Available: http://www.embase.com/search/results?subaction=viewrecord&from=export&id=L373978494%5Cnhttp://dx.doi.org/10.1186/1756-0500-7-155%5Cnhttp://sfx.hul.harvard.edu/sfx_local?sid=EMBASE&issn=17560500&id=doi:10.1186%2F1756-0500-7-155&atitle=Asymptomatic+urinary+

A. H. Mohamed, N. M. S. Omar, M. M. Osman, H. A. Mohamud, A. Eraslan, and M. Gur, “Antimicrobial Resistance and Predisposing Factors Associated with Catheter-Associated UTI Caused by Uropathogens Exhibiting Multidrug-Resistant Patterns: A 3-Year Retrospective Study at a Tertiary Hospital in Mogadishu, Somalia,” Trop. Med. Infect. Dis., vol. 7, no. 3, pp. 1–9, 2022, doi: 10.3390/tropicalmed7030042.

Y. A. Gessese et al., “Urinary pathogenic bacterial profile, antibiogram of isolates and associated risk factors among pregnant women in Ambo town, Central Ethiopia: A cross-sectional study,” Antimicrob. Resist. Infect. Control, vol. 6, no. 1, pp. 1–10, 2017, doi: 10.1186/s13756-017-0289-6.

A. Girma and A. Aemiro, “The Bacterial Profile and Antimicrobial Susceptibility Patterns of Urinary Tract Infection Patients at Pawe General Hospital, Northwest Ethiopia,” Scientifica (Cairo)., vol. 2022, no. 3, pp. 1–8, 2022, doi: 10.1155/2022/3085950.

B. S. Alsaywid et al., “Urinary tract infection in children: A narrative review of clinical practice guidelines,” Urol. Ann., vol. 15, no. 2, pp. 113–132, 2023, doi: 10.4103/ua.ua_147_22.

V. Folliero et al., “Prevalence and antimicrobial susceptibility patterns of bacterial pathogens in urinary tract infections in university hospital of campania ‘luigi vanvitelli’ between 2017 and 2018,” Antibiotics, vol. 9, no. 5, pp. 1–9, 2020, doi: 10.3390/antibiotics9050215.

C. Smoglica et al., “Antimicrobial Resistance Profile of Bacterial Isolates from Urinary Tract Infections in Companion Animals in Central Italy,” Antibiotics, vol. 11, no. 10, pp. 1–14, 2022, doi: 10.3390/antibiotics11101363.

S. Kumar, P. L. Kashyap, R. Singh, and A. K. Srivastava, “Preservation and Maintenance of Microbial Cultures,” in Analyzing Microbes, 2013, pp. 135–152. doi: 10.1007/978-3-642-34410-7_11.

M. N. A. Al-Muaala, S. Y. A. Al-Ethari, A. A. I. Al-Kraety, and G. S. Al-Muhanna, “Molecular Detection of Clumping factor A gene and Antibiotic Susceptibility Evaluation of Staphylococcus Aureus Isolated from Urinary Tract Infections,” Arch. Razi Inst., vol. 77, no. 2, pp. 573–578, 2022, doi: 10.22092/ARI.2022.357153.1985.

L. Deltourbe, L. Lacerda Mariano, T. N. Hreha, D. A. Hunstad, and M. A. Ingersoll, “The impact of biological sex on diseases of the urinary tract,” Mucosal Immunol., vol. 15, no. 5, pp. 857–866, 2022, doi: 10.1038/s41385-022-00549-0.

G. Gebremariam, H. Legese, Y. Woldu, T. Araya, K. Hagos, and A. Gebreyesuswasihun, “Bacteriological profile, risk factors and antimicrobial susceptibility patterns of symptomatic urinary tract infection among students of Mekelle University, northern Ethiopia,” BMC Infect. Dis., vol. 19, no. 1, pp. 1–11, 2019, doi: 10.1186/s12879-019-4610-2.

S. Malik, J. S. Rana, and K. Nehra, “Prevalence and Antibiotic Susceptibility Pattern of Uropathogenic Escherichia Coli Strains in Sonipat Region of Haryana in India,” Biomed. Biotechnol. Res. J., vol. 13, no. 3, pp. 80–87, 2021.

A. La Vecchia et al., “Epidemiology and antimicrobial susceptibility of Staphylococcus aureus in children in a tertiary care pediatric hospital in Milan, Italy, 2017—2021,” Ital. J. Pediatr., vol. 48, no. 1, pp. 1–8, 2022, doi: 10.1186/s13052-022-01262-1.

N. Ehiaghe, J. I, Amengialue, O.O, Ehiaghe, F.A, Maduka, “Plasmid Profile of Bacterial Isolates from Asymptomatic Bacteriuria among Undergraduate Students of a Tertiary Institution in Benin city, Nigeria,” Niger. J. Microbiol., vol. 34, no. 1, pp. 5007–5017, 2020.








How to Cite

Microbial Detection and Antibiotic  Susceptibility patterns  of Clinical Isolates from Women With Urinary Tract Infection in AL-Nasiriyah City/Iraq. (2024). University of Thi-Qar Journal of Science, 11(1), 45-54. https://doi.org/10.32792/utq/utjsci/v11i1.1168