Improvement of Newcastle disease virus vaccine by using gold nanoparticles and some natural food additives

Abstract

       Newcastle is a disease consider is one of important poultry infectious diseases, it  cause highly significance economic loss for the poultry industry. 120 one day age chicks is divided into sexgroups (A,B,C,D,E,k) two first group injected with Newcastle vaccine mixed with gold nanoparticles in different  concentrations, third group vaccinated then fed Cinnamon powder mixed with bird feed, forth group Nigella stava seeds mixed with birds feed, fifth group Cinnamon oil mixed with vaccine and the last group injected with  vaccine only which consider as control group. Antibodies against Newcastle vaccine and interferon gamma titer are estimated in a different period after vaccination 14, 21, 28 and 35 days by ELISA method. Highly antibodies titer were found in third group after 35 days (0.60 ± 0.07) while the two first groups were given significant results elevated of antibodies during all periods of experiment compare to other groups (0.54).Results showed that there were a significance difference between the groups (A, B, C, D, and E) and control group (K).The study revealed that highest IFN level titer was in group (B) which was (1.91), followed by groups (E, A, K). At  5 weeks old chicks , the highest  level of IFN  was in group (A)(gold nanoparticles-vaccine group 50:50) (1.91) , followed in group (E , B, C) was ( 1.64 , 1.62 , 1.61).Significant differences(p< 0.05) were found between different groups during experiment time